Title: | Genome-wide screening for complete genetic loss in prostate cancer by comparative hybridization onto cDNA microarrays. |
List of authors: | Clark J; Edwards S; Feber A; Flohr P; John M; Giddings I; Crossland S; Stratton MR; Wooster R; Campbell C; Cooper CS. |
Reference: | Oncogene 22:1247-52 |
Year of Publication: | 2003 |
ISSN: | 0950-9232 |
Keywords: | |
Abstract: | We demonstrate that comparative genomic hybridization (CGH) onto cDNA microarrays may be used to carry out genome-wide
screens for regions of genetic loss, including homozygous (complete) deletions that may represent the possible location of
tumour suppressor genes in human cancer. Screening of the prostate cancer cell lines LNCaP, PC3 and DU145 allowed the mapping
of specific regions where genome copy number appeared altered and led to the identification of two novel regions of complete
loss at 17q21.31 (500 kb spanning STAT3) and at 10q23.1 (50-350 kb spanning SFTPA2) in the PC3 cell line. |
PMID: | 12606952 |
Gene List: | ICR-geneset |
Supplementary data: | Supplementary data table (Excel format) |